• 2 “knocked-in” clones (both alleles) made with a single gRNA and 2 wild-type clones from the same pool of transfected cells that did not edit either allele;
• Strategy design and testing of up to 2 gRNAs;
• Mycoplasma test at arrival and before shipment;
• SNP or small tag oligo template;
• Electroporation of RNPs and GFP-encoding plasmid;
• Sorting GFP+ cells;
• Confirmation by Sanger sequencing that the locus is being targeted by the Cas9 endonuclease;
• Isolation of clones;
• Genotyping of clones and confirmation of knockout by Sanger sequencing;
• Clone expansion, blind reconfirmation and shipment;
• Maintenance of edited clones in our collection for six months, which can be resent for free (excluding post and package).

Extras:

• Cas9 “hifi”
• Extra single guide RNA
• Customized wild-type control
• Western-blot
• Real-time RT-qPCR

The price starts at 5500 euros.

• 2 vials of “knocked-in” bulk cultures;
• Strategy design and testing of up to 2 gRNAs;
• Mycoplasma test at arrival and before shipment;
• SNP or small tag oligo template;
• Electroporation of RNPs and GFP-encoding plasmid;
• Sorting GFP+ cells;
• Confirmation by Sanger sequencing that the locus is being targeted by the Cas9 endonuclease;
• Maintenance of edited bulks in our collection for six months, which can be resent for free (excluding post and package).

Extras:

• Cas9 “hifi”
• Extra single guide RNA
• Customized wild-type control
• Western-blot
• Real-time RT-qPCR

The price starts at 3100 euros.

“Do it yourself” is a kit for 4 transfections, consisting of:

• Editing strategy;
• 2 gRNAs;
• Template;
• Cas9;
• Tracer;
• Genotyping primers.

All kits are developed by our researchers based on the specifications of each project.
All “Do it yourself” kits are accompanied by online consultancy for 6 months.

Extras:

• Cas9 “hifi”
• Extra single guide RNA

The price starts at 1850 euros.

We can design the strategy from scratch or review your strategy within 3 days.