Crispr screens in pools allow you to identify genes involved in a given cell function. All we need is a library encoding sgRNAs for all or a subset of the transcriptome, usually in a lentiviral vector, and a cell assay that acts as a selection step. Once the cells have been transduced with the library, they undergo the selection step (for instance, exposure to a drug) and then we amplify and sequence the integrated sgRNA encoding regions from the cell pools before and after the selection. From the high-throughput sequencing data we can compare the frequencies of each individual sgRNA encoding region before and after the selection step. Any statistically significant difference (corrected for multiple comparisons) is likely to identify a gene that encodes a protein or RNA important for the selection step, by increasing the sensitivity or resistance of the cell.
Our Crispr screens start at 25k euros (turnaround time is 7 months) and include: